Join Our Team

Discover, Develop & Deliver


Our Culture

If you are considering a job with us, please take a few minutes to review our values. Operating with Integrity, Respect, Quality and Innovation are non-negotiable. At Obsidian, we embody the following values (obviously, we have a thing for alliterations):

Adaptability & Agility

We catalyze our evolution through continuous learning and sharing

Efficiency & Efficacy

We maximize our capabilities by leveraging each other’s expertise

Passion & Purpose

Our patients are waiting

Resilience & Results

We tenaciously overcome challenges to deliver meaningful outcomes

Camaraderie & Chemistry

We celebrate trust and community


We always welcome dedicated and talented applicants to apply to join our growing team. Please send resumes to jobs@obsidiantx.com; include a cover letter that highlights your qualifications and matches our requirements and please include the job title to which you are applying in the subject of the email.

Open Positions

Senior Research Associate, Cell Therapy Process Development

About Us…

Obsidian Therapeutics is pioneering the development of controllable cell and gene therapies to deliver transformative outcomes for patients with intractable diseases. Obsidian’s programs apply our CytoDriveTM technology in Cell and Gene therapy products to control expression of proteins for enhanced therapeutic efficacy and safety.

Our Opportunity…

We’re looking for a highly motivated Senior Research Associate with expertise in cell culture and upstream process development to contribute to developing and characterizing manufacturing processes for multiple cell therapy products in our pipeline targeting cancer. You will collaborate with the research groups to transfer and build on foundational know-how to deliver phase-appropriate manufacturing processes that meets the quality target profile. As part of our Technical Development and Manufacturing department, you will be a vital part of a highly collaborative team working to achieve Obsidian’s vision by translating innovative science into medical breakthroughs for patients.

This is a unique opportunity to be a scientific contributor in a well-funded early-stage company with blue-chip investors, field-leading advisors, and a highly experienced team. You will help us build a dynamic, innovative, passionate, collaborative, and successful organization focused on delivering transformative therapies in areas of greatest clinical need.

Located in the heart of Cambridge, we’re proud of our diverse talented team and committed to cultivating an environment of inclusion where each person feels a strong sense of belonging and is supported to continuously learn and contribute their best work. We offer competitive salary and benefits, and potential for employee ownership through stock options. Apply to jobs@obsidiantx.com.

You Will…

  • Plan and execute process development experiments with engineered T-cell and other immune cell product candidates to define process, optimize and scale-up for clinical manufacturing encompassing all steps of the process including starting material isolation, expansion, viral transduction, and cryopreservation
  • Perform in-process assays to measure cell number and characterize product using flow cytometry and other techniques
  • Interpret results, troubleshoot technical hurdles, and propose solutions to the team
  • Maintain excellent records of experiments in lab notebooks in a timely manner
  • Collaborate effectively across Obsidian organization, present internally to project teams, management, and scientific teams
  • Author and review technical reports detailing experimental work and summarizing results
  • Maintain a safe work environment in accordance with policies/procedures/regulations
  • Maintain cell cultures and cell banks as needed to support experimental work including occasional production of viral vectors
  • Support lab functions-including, but not limited to restocking consumables, maintaining equipment and cleaning

You Bring…

Core Qualifications

  • BS or MS degree in chemical engineering, biochemical engineering, biology or relevant field, with 2-6 years of experience in biotech/pharma setting
  • Strong experience with cell culture techniques with both adherent and suspension cell lines
  • Hands-on experience with plasmid transfection and viral transduction using lentiviral/retroviral vectors
  • Experience with analytical methods for cell characterization such as flow cytometry, quantitative PCR, western blot, and ELISA
  • Strong troubleshooting and communication skills
  • Strong record-keeping and organization skills, with an ability to manage multiple tasks in parallel
  • Highly collaborative work style
  • Ability to work independently and as part of teams to meet deadlines in a fast-paced environment, prioritize work across multiple projects, and adapt to changes in order to fulfill our mission

Bonus Qualifications

  • Experience with T-cell (or other immune cell) selection, viral transduction, gene editing technologies, cell expansion and cryopreservation
  • Basic knowledge of immunology and experience working with immune system cells
  • Experience with design of experiment (DOE) approaches and statistical analysis of data
  • Experience with the culture of mammalian cells in small -scale and large-scale bioreactor systems and associated control strategies and automation
  • Prior experience working with CDMOs, technology transfer in the context of process development to support GMP manufacturing
Senior Associate Scientist, Analytical Development

About Us…

Obsidian Therapeutics is pioneering controllable cell and gene therapies to deliver transformative outcomes for patients with intractable diseases. Obsidian’s programs apply our CytoDriveTM technology in Cell and Gene therapy products to control expression of proteins for enhanced therapeutic efficacy and safety. 

Our Opportunity…

We’re looking for a scientist with a strong background in analytical development to contribute to building Obsidian’s analytical sciences capabilities for process and product characterization, contributing to the first autologous cell therapy product in our pipeline targeting cancer. In this role, you’ll work collaboratively with the Manufacturing, Process Development, Regulatory, and Quality functions to develop analytical tools for the characterization of viral vectors and cell therapy products to support our clinical pipeline. As part of our Technical Development and Manufacturing department, you’ll be a vital part of a highly collaborative team working to achieve Obsidian’s vision by translating innovative science into medical breakthroughs for patients.

This is a unique opportunity in a well-funded early-stage company with blue chip investors, field-leading advisors, and a highly experienced team. You will help us build a dynamic, innovative, passionate, collaborative, and successful organization focused on delivering transformative therapies in areas of greatest clinical need.

Located in the heart of Cambridge, we’re proud of our diverse talented team and committed to cultivating an environment of inclusion where each person feels a strong sense of belonging and is supported to continuously learn and contribute their best work.  We offer competitive salary and benefits, and potential for employee ownership through stock options. Apply to jobs@obsidiantx.com.

You Will…

  • Apply your expertise in developing and qualifying analytical assays to support the release and characterization of viral vector and cell therapy products in early and later phases of development
  • Design, plan, and execute experiments in Analytical Development, working both independently and as part of a multidisciplinary team of researchers
  • Support development and/or improvement of methods in collaboration with research and vector process development to characterize viral vector production methods and primary cell transduction (eg T cell, TIL, NK cell)
  • Support introduction of new analytical technologies, implementation of common reagents and controls, trending of data, and protocols to support construct screening, research studies, research vector production, and CDMO tech transfer activities
  • Interpret results, troubleshoot technical hurdles, and propose solutions to the team
  • Maintain excellent records of experiments, including Electronic Notebook entries, authoring of protocols, test methods, and study reports
  • Collaborate effectively across Obsidian organization, present internally to project teams, management, and scientific teams

You Bring…

Core Qualifications

  • BS or MS in a relevant discipline (molecular biology, cell biology, biomolecular engineering, biotechnology or relevant field)
  • Minimum of 6-10 years relevant industry experience including prior direct experience with analysis of viral vectors and/or cell therapies
  • Demonstrated track record with analytical techniques focused on cell biology, immunology, molecular biology, and potency assay development
  • Subject matter expertise in analytical development, including methods such as multicolor flow cytometry/ FACS , cell line/ primary cell transduction/ transfection, , qPCR/ ddPCR, ELISA, MSD, and western blot
  • Strong troubleshooting and communication skills.
  • Highly organized with strong record keeping and an ability to manage multiple tasks in parallel
  • Highly collaborative working style and ability to adapt in a fast paced, rapidly developing environment
  • Ability to work with general direction to meet objectives and timelines, adapting to changes and priorities in order to fulfill our mission

Bonus Qualifications

  • Experience with development of flow cytometry-based assays for release and characterization
  • Experience in analytical development supporting early and/ or late stage development of biologics is highly desirable (cell therapy, gene therapy, vaccines, antibodies)
  • Demonstrated track record with analytical development and method qualification for characterization of engineered cell therapies
  • Familiarity with viral vector production and cell line engineering
  • Experience with Design of Experiment (DOE) approaches and application to analytical development
  • Experience working with CDMOs/ CTLs for method transfer/ development/ qualification/ validation
  • Familiarity with FDA and EMA guidance documents relevant to gene and cell therapy analytical development and method validation
Senior Associate Scientist, Vector Process Development

About Us…

Obsidian Therapeutics is pioneering controllable cell and gene therapies to deliver transformative outcomes for patients with intractable diseases. Obsidian’s programs apply our CytoDriveTM technology in Cell and Gene therapy products to control expression of proteins for enhanced therapeutic efficacy and safety.

Our Opportunity…

We’re looking for a highly motivated scientist with a strong background in process development to contribute to building Vector Process Development capabilities for Obsidian to drive the development of the engineered cell therapy products in our pipeline targeting cancer. In this role, you’ll work with the Manufacturing, Cell Therapy Process Development, Analytical Development, Regulatory, and Quality functions to develop gene delivery processes to support Obsidian’s engineered cell therapy clinical pipeline. As part of our Technical Development and Manufacturing department, you will be a vital part of a highly collaborative team working to achieve Obsidian’s vision by translating innovative science into medical breakthroughs for patients.

This is a unique opportunity in a well-funded early-stage company with blue-chip investors, field-leading advisors, and a highly experienced team. You will help us build a dynamic, innovative, passionate, collaborative, and successful organization focused on delivering transformative therapies in areas of greatest clinical need.

Located in the heart of Cambridge, we’re proud of our diversely talented team and committed to cultivating an environment of inclusion where each person feels a strong sense of belonging and is supported to continuously learn and contribute their best work. We offer competitive salary and benefits, and potential for employee ownership through stock options. Apply to jobs@obsidiantx.com.

You Will…

  • Apply your expertise in the development of processes for viral vector production to support engineered cell therapies.
  • Assist with developing scalable and efficient viral vector production processes and materials for engineered cell therapies.
  • Design, plan, and execute experiments in Vector Process Development, working both independently and as part of a multidisciplinary team of researchers
  • Identify and implement solutions to address current technical challenges in viral vector (eg lentiviral and retroviral) production processes in collaboration with research to support engineered cell therapies
  • Run and/or improve methods in collaboration with research and analytical development to characterize viral vector production methods and primary cell transduction
  • Supply viral vector materials to support research studies, analytical development, and CDMO tech transfer activities
  • Interpret results, troubleshoot technical hurdles, and propose solutions to the team
  • Maintain excellent records of experiments, including Electronic Notebook entries, authoring of protocols, test methods, and study reports
  • Collaborate effectively across the Obsidian organization, present internally to project teams, management, and scientific teams
  • Maintain a safe work environment in accordance with policies/procedures/regulations

You Bring…

Core Qualifications

  • BS or MS in a relevant discipline (eg chemical engineering, cellular and molecular biology, biotechnology)
  • Minimum of 6-10 years relevant industry experience including prior direct experience in viral vector biology and/or production
  • Demonstrated track record with different gene delivery methodologies, such as lentivirus/ retrovirus/ AAV supporting early-stage research and development of biologics
  • Subject matter expertise in mammalian cell culture, including adherent and suspension cell culture scale-up/down and stable cell line development
  • Subject matter expertise in viral vector production, including downstream purification and concentration methods (eg Tangential Flow Filtration)
  • Familiar with product characterization methods relevant to viral vectors (e.g. cell line/primary cell transduction, titering, flow cytometry, qPCR/ ddPCR, ELISA, MSD, and western blot)
  • Strong troubleshooting and communication skills
  • Highly organized with strong record-keeping skills, and an ability to manage multiple tasks in parallel
  • Highly collaborative working style
  • Ability to work independently and as part of teams to meet deadlines in a fast-paced rapidly developing environment, prioritize work across multiple projects, and adapt to changes in order to fulfill our mission

Bonus Qualifications

  • Experience in process development supporting early and/ or late-stage development of biologics is highly desirable (cell therapy, gene therapy, vaccines, antibodies)
  • Experience with Design of Experiment (DOE) approaches and application to process improvement
  • Experience in technical transfer of processes to CDMOs
  • Familiarity with FDA and EMA guidance documents relevant to gene and cell therapy is a plus
Senior Scientist, Analytical Development

About Us…

Obsidian Therapeutics is pioneering controllable cell and gene therapies to deliver transformative outcomes for patients with intractable diseases. Obsidian’s programs apply our CytoDriveTM technology in Cell and Gene therapy products to control expression of proteins for
enhanced therapeutic efficacy and safety.

Our Opportunity…

We’re looking for a leader in analytical development to contribute to building Obsidian’s analytical sciences capabilities for process and product characterization, driving the development of the first autologous cell therapy product in our pipeline targeting cancer. This is a key role, focusing on molecular and immunological assay development. You’ll work collaboratively with the Analytical Development team as well as Manufacturing, Process Development, Regulatory, and Quality functions to develop analytical tools for the characterization of viral vectors and cell therapy products to support our clinical pipeline. As part of our Technical Development and Manufacturing department, you’ll be a vital part of working to achieve Obsidian’s vision by collaboratively translating innovative science into medical breakthroughs for patients.

This is a unique opportunity in a well-funded early-stage company with blue-chip investors, field-leading advisors, and a highly experienced team. You will help us build a dynamic, innovative, passionate, collaborative, and successful organization focused on delivering transformative therapies in areas of greatest clinical need.

Located in the heart of Cambridge, we’re proud of our diverse talented team and committed to cultivating an environment of inclusion where each person feels a strong sense of belonging and is supported to continuously learn and contribute their best work. We offer competitive salary and benefits, and potential for employee ownership through stock options. Apply to jobs@obsidiantx.com.

You Will…

  • Apply your expertise in developing and qualifying analytical assays to support the release and characterization of viral vector and cell therapy products in early and later phases of development.
  • Design, plan, and execute experiments in Analytical Development, working both independently and as part of a multidisciplinary team of researchers
  • Support method development and provide technical expertise for the qualification of assays to assess identity, purity, safety and potency in engineered primary cells (eg TIL, T Cell, NK cell) and viral vectors (eg lentiviral vectors)
  • Support introduction of new analytical technologies, implementation of common reagents and controls, trending of data, and generation of SOPs to support vector production, and transfer of analytical methods to CDMOs
  • Interpret results, troubleshoot technical hurdles, and propose solutions to the team
  • Maintain excellent records of experiments, including Electronic Notebook entries.
  • Author and review technical and scientific documents including but not limited to study protocols and reports, validation documents, SOPs, and test methods
  • Help build the team and mentor/train team members on experiment design, data analysis with adequate scientific rigor and proper documentation in lab notebooks and reports
  • Collaborate effectively across Obsidian organization, present internally to project teams, management, and scientific teams.
  • Represent analytical development in CMC sub-teams for assigned projects and support project activities including authoring of relevant CMC sections in regulatory documents

You Bring….

Core Qualifications

  • Ph.D. in a relevant discipline (immunology, biochemistry, cell biology, biotechnology or related field) and 3+ years relevant industry experience. or MS and 10+ years of relevant industry experience.
  • Prior direct experience in viral vectors or cell therapy
  • Demonstrated track record with analytical development supporting early and/or late-stage development of biologics
  • Subject matter expertise in product characterization methods relevant to an immune cell-based product and viral vector starting materials, with a focus on molecular and immunoassay development (PCR / qPCR, ddPCR, ELISA, MSD and western blot)
  • Strong troubleshooting and communication skills, highly organized with strong record-keeping and an ability to multi-task
  • Highly collaborative working style and ability to adapt in a fast paced, rapidly developing environment
  • Ability to work with minimal direction to meet objectives and timelines, adapting to changes and priorities in order to fulfill our mission

Bonus Qualifications

  • Demonstrated track record with analytical development and method qualification for characterization of engineered cell therapies or viral vectors
  • Experience with multi-parameter flow cytometry assays, cell line/ primary cell transduction/ transfection, and cell-based assay development
  • Experience with Design of Experiment (DOE) approaches and application to analytical development
  • Experience working with CDMOs/ CTLs for method transfer/ development/ qualification/ validation
  • Familiarity with FDA and EMA guidance documents relevant to gene and cell therapy analytical development and method validation
Senior Scientist, Cell Therapy Process Development

About Us…

Obsidian Therapeutics is pioneering the development of controllable cell and gene therapies to deliver transformative outcomes for patients with intractable diseases. Obsidian’s programs apply our CytoDriveTM technology in Cell and Gene therapy products to control expression of proteins for enhanced therapeutic efficacy and safety.

Our Opportunity…

We’re looking for a highly motivated scientist with a strong background in cell therapy and/or process development to help drive the development of multiple cell therapy products in our pipeline targeting cancer. As part of our Technical Development and Manufacturing department, you will be a vital part of a highly collaborative team working to achieve Obsidian’s vision by translating innovative science into medical breakthroughs for patients.

This is a unique opportunity in a well-funded early-stage company with blue-chip investors, field-leading advisors, and a highly experienced team. You will help us build a dynamic, innovative, passionate, collaborative, and successful organization focused on delivering transformative therapies in areas of greatest clinical need.

Located in the heart of Cambridge, we’re proud of our diverse talented team and committed to cultivating an environment of inclusion where each person feels a strong sense of belonging and is supported to continuously learn and contribute their best work. We offer competitive salary and benefits, and potential for employee ownership through stock options. Apply to jobs@obsidiantx.com.

You Will…

  • Contribute your expertise to develop clinical/commercial manufacturing processes, cell therapy products, tech transfer to CDMOs, oversight of external manufacturing, and regulatory filings
  • Drive the design and execution of experiments for development of the cGMP manufacturing process and associate process controls for clinical supply of cell therapy product (and related materials) meeting yield and quality targets
  • Author and review technical and scientific documents including but not limited to study protocols and reports, validation documents, SOPs, and test methods
  • Help build the team, and train and mentor team members on experiment design, data analysis with appropriate scientific rigor and proper documentation in lab notebooks
  • Innovate solutions to address current technical challenges and evaluate/ incorporate next-generation process technologies
  • Supply development materials (viral vector, cell product) to support research/nonclinical studies, analytical development, and CDMO tech transfer activities
  • Lead and support key technology transfer document preparation, technology transfer & implementation of the process, and serve as the technical point of contact for the process development function in assigned projects
  • Establish strong relationships and collaborate closely with internal stakeholders (research, quality, regulatory, project management)
  • Represent process development in CMC sub-teams for assigned projects and support project activities including authoring of relevant CMC sections in regulatory documents

You Bring…

Core Qualifications

  • Ph.D. and 3+ years of industry experience in a relevant discipline (chemical engineering, biomolecular engineering, biotechnology or relevant field), or MS plus 10 years relevant experience.
  • Subject matter expertise in mammalian cell culture and related aspects such as cell metabolism/media optimization, engineering fundamentals on cell culture scaleup/down, cell biology/physiology
  • Hands-on experience with product characterization methods relevant to cell therapy (e.g. flow cytometry)
  • Highly collaborative working style, and ability to adapt in a fast-paced, rapidly developing environment
  • Ability to work with minimal direction to meet objectives and timelines, adapting to changes and priorities in order to fulfill our mission

Bonus Qualifications

  • Prior experience with T-cells, NK cells, induced pluripotent stem cells (iPSC), or other primary cells is a plus.
  • Demonstrated track record with process development supporting early and/or late-stage development of biologics (cell therapy, gene therapy, vaccines, antibodies…)
  • Experience with and technology transfer to CDMOs and external manufacturing oversight
  • Familiar with FDA and EMA guidance documents relevant to gene and cell therapy
  • Experience authoring CMC sections of regulatory documents (IND/BLA) and engaging regulatory authorities
  • Experience with Design of Experiment (DOE) approaches and application to process development
  • Strong working knowledge of cGMP and quality systems requirements
  • Prior experience in managing people and matrixed teams
Senior Director, Process Development

About Us…

Obsidian Therapeutics is pioneering the development of controllable cell and gene therapies to deliver transformative outcomes for patients with intractable diseases. Obsidian’s programs apply our CytoDriveTM technology in Cell and Gene therapy products to control the expression of proteins for enhanced therapeutic efficacy and safety.

Our Opportunity…

We’re looking for a leader in CMC to build process development capabilities for Obsidian to drive the development of autologous and allogeneic cell therapy products in our pipeline targeting cancer. The Senior Director of Process Development is a key leadership role within Technical Development and Manufacturing; you will have significant opportunity to contribute to, and lead the execution of, Obsidian’s process development strategy as well as other development strategies and efforts. You’ll collaborate with the Manufacturing, Analytical Development, Regulatory, and Quality functions to develop and tech transfer robust manufacturing processes and provide oversight of external manufacturing of viral vector and cell therapy drug substance/drug products to support Obsidian’s clinical pipeline. As part of our Technical Development and Manufacturing department, you will be a vital part of a highly collaborative team working to achieve Obsidian’s vision by translating innovative science into medical breakthroughs for patients.

This is a unique opportunity in a well-funded early-stage company with blue-chip investors, field-leading advisors, and a highly experienced team. You will help us build a dynamic, innovative, passionate, collaborative, and successful organization focused on delivering transformative therapies in areas of greatest clinical need.

Located in the heart of Cambridge, we’re proud of our diverse talented team and committed to cultivating an environment of inclusion where each person feels a strong sense of belonging and is supported to continuously learn and contribute their best work. We offer competitive salary and benefits, and potential for employee ownership through stock options. Apply to jobs@obsidiantx.com.

You Will…

  • Apply your expertise in leading the development of clinical and commercial manufacturing processes for viral vector and cell therapy products, tech transfer to CDMOs, and oversight to external manufacturing. Develop, tech transfer and implement cGMP manufacturing processes capable of supplying clinical trials with viral vector and cell therapy product (and related materials) meeting quality targets, and regulatory requirements
  • Identify and implement solutions to address current technical challenges in the manufacturing process and support immediate manufacturing needs and innovate to enable next-generation process engineering
  • Develop and/or improve methods in collaboration with research and analytical development to characterize cell function relevant phenotype to support rigorous approach to evaluate the impact of process changes
  • Supply development materials (viral vector, cell product) to support research/nonclinical studies, analytical development, and CDMO tech transfer activities
  • Author and review technical and scientific documents including but not limited to study protocols and reports, validation documents, SOPs, and test methods
  • Establish strong relationships across multiple functions within Obsidian as well as externally with Obsidians strategic partners.
  • Collaborate effectively with research, CMC team, and CDMO to support IND enabling activities
  • Act as subject matter expert and represent the process development function as needed for regulatory interaction and author relevant CMC sections in regulatory documents
  • Recruit talent, enable, coach and develop talent as needed and manage process development function
  • Define the agenda and manage experimental activities in the process development function to meet company goals
  • Ensure team follows proper lab safety protocols and maintains proper documentation and quality scientific documents including but not limited to Electronic Notebooks, study protocols and reports, validation documents, SOPs, and test methods

You Bring…

Core Qualifications

  • Ph.D. in a relevant discipline (chemical engineering, biomolecular engineering, biotechnology or relevant field)
  • Minimum 10 years of relevant industrial experience with prior direct experience in viral vectors and/or cell therapy required
  • Demonstrated track record with process development supporting early and late-stage development of biologics (cell therapy, gene therapy, vaccines, antibodies…)
  • Experience leading and managing teams, cultivating an inclusive climate and engaging and developing talent to their individual and collective potential
  • Experience working with internal and external stakeholders to enable regulatory submissions.
  • Experience with technology transfer to CDMOs, and external manufacturing oversight
  • Subject matter expertise in mammalian cell culture and related aspects such as cell metabolism/media optimization, engineering fundamentals on cell culture scaleup/down, cell biology/physiology
  • Familiar with product characterization methods relevant to immune cell-based product (e.g. flow cytometry, T-cell phenotyping, cell function characterization assays, PCR/qPCR, ELISA, and western blot) and/or viral vector manufacturing.
  • Familiar with FDA and EMA guidance documents relevant to gene and cell therapy
  • Experience authoring CMC sections of regulatory documents (IND/BLA) and engaging regulatory authorities.
  • Strong working knowledge of cGMP and quality systems requirements.
  • Highly collaborative working style, and ability to lead and adapt in a fast-paced, rapidly developing environment
  • Ability to work with minimal direction to meet objectives and timelines, adapting to changes and priorities in order to fulfill our mission
  • Demonstrated ability to build process development organization
  • Ability to effectively influence others.
  • Prior experience representing function as a leader in CMC teams

Bonus Qualifications

  • Prior experience with T cells, NK cells, induced pluripotent stem cells (IPSC), or other primary cells is highly desirable