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Our Culture

If you are considering a job with us, please take a few minutes to review our values. Operating with Integrity, Respect, Quality and Innovation are non-negotiable. At Obsidian, we embody the following values (obviously, we have a thing for alliterations):

Adaptability & Agility

We catalyze our evolution through continuous learning and sharing

Efficiency & Efficacy

We maximize our capabilities by leveraging each other’s expertise

Passion & Purpose

Our patients are waiting

Resilience & Results

We tenaciously overcome challenges to deliver meaningful outcomes

Camaraderie & Chemistry

We celebrate trust and community

 

“Obsidian provides the opportunity to collaborate in teams, with an open exchange of ideas and learnings. This transparency and curiosity fosters individual professional development while advancing the team toward developing new therapeutics with the potential to impact patient lives.”


Jennifer Gori, Ph.D.
Director of Program Strategy
 

We always welcome dedicated and talented applicants to apply to join our growing team. Please send resumes to jobs@obsidiantx.com; include a cover letter that highlights your qualifications and matches our requirements.

Open Positions

Sr. Scientist, Cell Therapy

About the Company:

Obsidian Therapeutics is a biotechnology company that is pioneering the development of controllable cell and gene therapies to deliver transformative outcomes for patients with intractable diseases.  Obsidian’s lead programs are in adoptive immune cell therapy, applying our cytoDRiVE™ technology to control the expression of proteins for enhanced therapeutic efficacy and safety.

We are looking for a talented, flexible, collaborative, dynamic scientist to join the department of Cell Therapy as key contributors to preclinical research supporting Cell Therapy program research and development.

This role is a unique opportunity to at a well-funded startup with blue chip investors, field-leading founders and advisors, and a highly experienced biotech team. You will help us to continue to build a transparent and successful organization focused on delivering transformative therapies in oncology and other areas of greatest clinical need. Located in an energetic environment in the heart of Cambridge, Obsidian is an equal opportunity employer offering a competitive salary and benefits package. Resumes can be sent to jobs@obsidiantx.com.

Primary Responsibilities:

  • Lead preclinical proof of concept research activities toward the development of cell therapy products
  • Mentor and/or mentor a team of scientists/research associates in the design, execution and analysis of experiments that support programs
  • Design, develop and apply cell-based immune assays, including engineering of human T and NK cells
  • Make independent, intellectual, and technical contributions to projects, while working on a collaborative team
  • Isolate immune cells from human blood and tissue and conduct ex vivo engineering, differentiation, characterization, and/or in vivo adoptive cell transfer studies
  • Independently design experiments, meticulously execute bench work, troubleshoot assays, analyze data, and communicate findings
  • Present results at internal meetings

Required Qualifications and Preferred Skills:

  • PhD in Immunology or a related field with 3+ years of industry experience
  • Ability to quickly learn and adapt to fast paced, rapidly changing start-up environment
  • Scientific rigor in the design, execution, analysis, and troubleshooting of scientific experiments
  • Experience leading and driving research projects
  • Highly motivated team contributor, with ability to work independently and collaboratively
  • Excellent organization, record keeping, multi-tasking, and communication skilled
  • Demonstrated expertise in ex vivo immunology methods including T and NK cell genetic engineering (gene transfer or gene editing), functional assay development using techniques such as multi-color flow cytometry, cytotoxicity assays, cytokine quantitation
  • Experience with tumor infiltrating lymphocytes (TIL) preferred
  • Viral vector transduction of primary human immune cells preferred
  • Cellular immunotherapy experience preferred
  • Authorized to work in the US at the time of application
Associate Scientist/ Senior Associate Scientist Vector Process Development

Obsidian Therapeutics is pioneering controllable cell and gene therapies to deliver transformative outcomes for patients with intractable diseases. We are looking for a scientist with a strong background in process development in our Technical Operations Department to contribute to building Vector Technology capabilities for Obsidian driving the development of the engineered cell therapy products in our pipeline targeting cancer. You will be a vital part of helping to achieve Obsidian’s vision by collaboratively translating innovative science into medical breakthroughs for patients.

Scope:

The Associate Scientist/ Senior Associate Scientist in Vector Process Development is a key role within our Technical Operations function and will be responsible for assisting with developing scalable and efficient viral vector production processes and materials for engineered cell therapies. The individual in this role will work collaboratively with the Manufacturing, Cell Process Development, Analytical Development, Regulatory, and Quality functions to develop gene delivery processes to support Obsidian’s engineered cell therapy clinical pipeline.

Key Responsibilities

The ideal candidate would have expertise in in the development of processes for viral vector production to support engineered cell therapies. In addition, the candidate should have experience in managing teams and working with internal and external stakeholders in a highly matrixed fast paced environment.

  • Identify and implement solutions to address current technical challenges in viral vector (eg lentivirus and retrovirus) research and development production processes to support engineered cell therapies
  • Serve as subject matter expert and support technology transfer as needed to viral vector CDMOs for cGMP clinical production
  • Support immediate cell therapy needs for gene delivery while innovating to enable next generation gene delivery technologies
  • Develop and/or improve methods in collaboration with research and analytical development to characterize viral vector production methods and primary cell transduction
  • Supply viral vector materials to support research studies, analytical development, and CDMO tech transfer activities
  • Author and review technical and scientific documents including but not limited to ELN entries, study protocols and reports, SOPs and test methods
  • Communicate effectively through presentations and document work clearly through written reports

Minimum Qualifications

  • BS or MS in a relevant discipline (eg chemical engineering, cellular and molecular biology, biotechnology)
  • Minimum of 3 years relevant industrial experience with prior direct experience in viral vector biology and/or production
  • Demonstrated track record with different gene delivery methodologies, such as lentivirus/ retrovirus/ AAV supporting early stage research and development of biologics
  • Subject matter expertise in mammalian cell culture, including adherent and suspension cell culture scale-up/down and stable cell line development
  • Subject matter expertise in viral vector production, including downstream purification and concentration methods (eg Tangential Flow Filtration)
  • Familiar with product characterization methods relevant to viral vectors (e.g. cell line/ primary cell transduction, titering, flow cytometry, qPCR/ ddPCR, ELISA, MSD, and western blot)
  • Ability to work in a fast-paced, collaborative environment, working with minimal direction and able to manage workload based on changing priorities.
  • Strong troubleshooting and communication skills, highly organized with strong record keeping and an ability to multi-task
  • Highly collaborative working style and ability to adapt in a fast paced, rapidly developing environment

Preferred Qualifications

  • Experience in process development supporting early and/ or late stage development of biologics is highly desirable (cell therapy, gene therapy, vaccines, antibodies)
  • Experience with Design of Experiment and application to process improvement
  • Familiarity with FDA and EMA guidance documents relevant to gene and cell therapy is a plus
Senior Associate Scientist/ Scientist Analytical Development

Obsidian Therapeutics is pioneering controllable cell and gene therapies to deliver transformative outcomes for patients with intractable diseases. We are looking for a scientist with a strong background in analytical development  to contribute to building Obsidian’s analytical sciences capabilities for process and product characterization within the Technical Operations department driving the development of the first autologous cell therapy product in our pipeline targeting cancer. You will be a vital part of helping to achieve Obsidian’s vision by collaboratively translating innovative science into medical breakthroughs for patients.

Scope:

The Senior Associate Scientist/ Scientist in Analytical Development is a key role within our Technical Operations function and will be responsible for assisting with establishing the company’s analytical sciences capabilities. The individual in this role will work collaboratively with the Manufacturing, Process Development, Regulatory, and Quality functions to develop analytical tools for the characterization of viral vectors and cell therapy products to support Obsidian’s clinical pipeline.

Responsibilities

The ideal candidate would have expertise in developing and qualifying analytical assays to support the release and characterization of viral vector and cell therapy products in early and later phases of development.

  • Design, plan, and execute experiments in Analytical Development, working both independently and as part of a multidisciplinary team of researchers
  • Develop and/or improve methods in collaboration with research and vector technologies to characterize viral vector production methods and primary cell transduction (eg T cell, TIL, NK cell)
  • Support introduction of new analytical technologies, implementation of common reagents and controls, trending data, and protocols to support construct screening, research studies, research vector production, and CDMO tech transfer activities
  • Interpret results, troubleshoot technical hurdles, and propose solutions to the team
  • Maintain excellent records of experiments, including Electronic Notebook entries, authoring of protocols, test methods, and study reports
  • Collaborate effectively across Obsidian organization, present internally to project teams, management, and scientific teams

Minimum Qualifications

  • MS or Ph. D. in a relevant discipline (molecular biology, cell biology, biomolecular engineering, biotechnology or relevant field)
  • Minimum of 5-7 years (MS) or 1-3 years (PhD) relevant industrial experience with prior direct experience with analysis of viral vectors and/or cell therapies required
  • Demonstrated track record with analytical techniques focused on cell biology, immunology, molecular biology, and potency assay development
  • Subject matter expertise in analytical development, including methods such as cell line/ primary cell transduction/ transfection, multicolor flow cytometry/ FACS, qPCR/ ddPCR, ELISA, MSD, and western blot
  • Experience in analytical development supporting early and/ or late stage development of biologics is highly desirable (cell therapy, gene therapy, vaccines, antibodies)
  • Strong troubleshooting and communication skills, highly organized with strong record keeping and an ability to multi-task
  • Highly collaborative working style and ability to adapt in a fast paced, rapidly developing environment

Preferred Qualifications

  • Demonstrated track record with analytical development and method qualification for characterization of engineered cell therapies
  • Familiarity with viral vector production and cell line engineering
  • Experience with Design of Experiment and application to analytical development
  • Experience working with CDMOs/ CTLs for method transfer/ development/ qualification/ validation
  • Familiarity with FDA and EMA guidance documents relevant to gene and cell therapy analytical development and method validation
Senior/ Principal Scientist Vector Process Development

Obsidian Therapeutics is pioneering controllable cell and gene therapies to deliver transformative outcomes for patients with intractable diseases. We are looking for a leader in our Technical Operations Department to develop Vector Technology capabilities for Obsidian driving the development of the engineered cell therapy products in our pipeline targeting cancer. You will be a vital part of helping to achieve Obsidian’s vision by collaboratively translating innovative science into medical breakthroughs for patients.

Scope:

The Senior Scientist/ Principal Scientist in Vector Process Development is a key leadership role within our Technical Operations function and will be responsible for leading the Vector Technologies group. The Vector Technologies group is responsible for developing scalable and efficient viral vector production processes and materials for the development of engineered cell therapies. The individual in this role will work collaboratively with the Manufacturing, Cell Process Development, Analytical Development, Regulatory, and Quality functions to develop gene delivery processes to support Obsidian’s engineered cell therapy clinical pipeline.

Key Responsibilities

The ideal candidate would have expertise in in the development of processes for viral vector production to support engineered cell therapies. In addition, the candidate should have experience in managing teams and working with internal and external stakeholders in a highly matrixed fast paced environment.

  • Identify and implement solutions to address current technical challenges in viral vector (eg lentivirus and retrovirus) research and early development production processes to support engineered cell therapies
  • Serve as subject matter expert and support technology transfer as needed to viral vector CDMOs for cGMP clinical production
  • Support immediate cell therapy needs for gene delivery while innovating to enable next generation gene delivery technologies
  • Develop and/or improve methods in collaboration with research and analytical technologies to characterize viral vector production methods and primary cell transduction
  • Lead supply of viral vector materials to support research studies, analytical development, and CDMO tech transfer activities
  • Author and review technical and scientific documents including but not limited to ELN entries, study protocols and reports, SOPs and test methods
  • Establish strong relationships and collaborate closely across multiple functions, including discovery, Cell therapy, CMC and translational research within Obsidian as well as externally with Obsidians strategic partners.
  • Recruit talent as needed and manage Vector Technologies function with needed capabilities
  • Define agenda and manage experimental activities in the Vector Technologies function and ensure proper documentation

Minimum Qualifications

  • D. in a relevant discipline (eg chemical engineering, cellular and molecular biology, biotechnology)
  • Minimum of 3-8 years relevant industrial experience with prior direct experience in viral vector biology and/or production
  • Demonstrated track record with different gene delivery methodologies, such as lentivirus/ retrovirus/ AAV supporting early stage research and development of biologics
  • Subject matter expertise in mammalian cell culture, including adherent and suspension cell culture scale-up/down and stable cell line development
  • Subject matter expertise in viral vector production, including downstream purification and concentration methods (eg Tangential Flow Filtration)
  • Familiar with product characterization methods relevant to viral vectors (e.g. cell line/ primary cell transduction, titering, flow cytometry, qPCR/ ddPCR, ELISA, MSD, and western blot)
  • Experience managing laboratory scientists
  • Familiarity with FDA and EMA guidance documents relevant to gene and cell therapy
  • Ability to work in a fast-paced, collaborative environment, working with minimal direction and able to manage workload based on changing priorities.
  • Demonstrated leadership skills and the ability to collaborate with and effectively influence others.

Preferred Qualifications

  • Experience in process development supporting early and/ or late stage development of biologics is highly desirable (cell therapy, gene therapy, vaccines, antibodies)
  • Experience in primary cell gene delivery, including cell types such as T cells, TILs, NK cells
  • Experience with Design of Experiment and application to process improvement
  • Experience with and technology transfer to CDMOs and external manufacturing oversight
  • Prior experience managing functional teams and representing function in cross-functional teams
  • Experience authoring CMC sections of regulatory documents (IND/BLA) and engaging regulatory authorities.
Senior Scientist/Principal Scientist, Analytical Development

Obsidian Therapeutics is pioneering controllable cell and gene therapies to deliver transformative outcomes for patients with intractable diseases. We are looking for a leader in analytical development to contribute to building Obsidian’s analytical sciences capabilities for process and product characterization within the Technical Operations department driving the development of the first autologous cell therapy product in our pipeline targeting cancer. You will be a vital part of helping to achieve Obsidian’s vision by collaboratively translating innovative science into medical breakthroughs for patients.

The Senior Scientist/Principal Scientist, Analytical Development is a key role within our Technical Operations function and will be responsible for assisting with establishing the company’s analytical sciences capabilities. The individual in this role will work collaboratively with the Manufacturing, Process Development, Regulatory, and Quality functions to develop analytical tools for the characterization of viral vectors and cell therapy products to support Obsidian’s clinical pipeline.

Responsibilities

The ideal candidate would have expertise in developing and qualifying analytical assays to support the release and characterization of viral vector and cell therapy products in early and later phases of development.

  • Responsible for the development, optimization, qualification/validation of analytical methods for product characterization and release for viral vector and/or engineered cell therapy products (and related materials).
  • Author and review technical and scientific documents including but not limited to study protocols and reports, validation documents, SOPs, and test methods
  • Help build the team and mentor/train team members on experiment design, data analysis with adequate scientific rigor and proper documentation in lab notebooks
  • Evaluation of new technologies relevant to product characterization for viral vectors and cell therapy drug products
  • Develop cell-based assays to assess potency for viral vectors and cell therapy drug products
  • Lead/support analytical method protocol preparation, analytical method transfer & implementation of the method in a CDMO (within QC) or CRO, and serve as the technical point of contact for the analytical development function in assigned projects
  • Establish strong relationships and collaborate closely with Obsidian stakeholders (research, quality, regulatory, project management) and provide analytical support (to process development, research, and other group in support IND enabling activities
  • Represent the Technical Operations function in project teams and lead CMC/ functional sub teams for assigned projects and support project activities including authoring of relevant CMC sections in regulatory documents

Qualifications

  • D. in a relevant discipline (immunology, biochemistry, cell biology, biotechnology or related field)
  • Minimum 3-8 years relevant industrial experience with prior direct experience in viral vectors or cell therapy required
  • Demonstrated track record with analytical development supporting early and/or late stage development of biologics
  • Subject matter expertise in product characterization methods relevant to immune cell-based product (e.g. flow cytometry, T-cell phenotyping, cell function characterization assays, PCR / qPCR, ddPCR, ELISA, MSD and western blot) and/or viral vector manufacturing.
  • Familiar with FDA and EMA guidance documents relevant to gene and cell therapy
  • Experience authoring CMC sections of regulatory documents. Working knowledge of quality systems requirements.
  • Ability to work in a fast-paced, collaborative environment, working with minimal direction and able to manage workload based on changing priorities.
  • Ability to collaborate with and effectively influence others.
  • Prior experience managing functional teams and representing function in CMC teams
Senior Research Associate / Senior Associate Scientist: Cellular Immunology

Obsidian Therapeutics is a biotechnology company that is pioneering the development of controllable cell and gene therapies to deliver transformative outcomes for patients with intractable diseases.  Obsidian’s lead programs are in adoptive immune cell therapy, applying our cytoDRiVE™ technology to control the expression of proteins for enhanced therapeutic efficacy and safety.

We are looking for a talented, flexible, collaborative, dynamic scientist to join the department of Cell Therapy as key contributors to preclinical research supporting Cell Therapy program research and development.

This role is a unique opportunity to at a well-funded startup with blue chip investors, field-leading founders and advisors, and a highly experienced biotech team. You will help us to continue to build a transparent and successful organization focused on delivering transformative therapies in oncology and other areas of greatest clinical need. Located in an energetic environment in the heart of Cambridge, Obsidian is an equal opportunity employer offering a competitive salary and benefits package. Resumes can be sent to jobs@obsidiantx.com.

Primary Responsibilities:

  • Make independent intellectual and technical contributions to projects, while working on a collaborative team
  • Design, develop and apply cell-based immune assays, including engineering of human T and NK cells
  • Isolate immune cells from human blood and tissue and conduct ex vivo engineering, differentiation, characterization, and/or in vivo adoptive cell transfer studies
  • Perform ex vivo analysis of samples from in vivo adoptive cell transfer studies, including immunophenotyping and serum analysis
  • Independently design experiments, meticulously execute bench work, troubleshoot assays, analyze data, and communicate findings
  • Present results at internal meetings

Required Qualifications and Preferred Skills:

  • BS/MS with 3+ yrs experience in an industry setting (job title will depend on level of experience)
  • Ability to quickly learn and adapt to fast paced, rapidly changing start-up environment
  • Scientific rigor in the design, execution, analysis, and troubleshooting of scientific experiments
  • High level of motivation and ability to work both independently and collaboratively
  • Excellent organization, record keeping, multi-tasking, and communication skills
  • Demonstrated expertise in ex vivo immunology methods including T and NK cell genetic engineering (gene transfer or gene editing), functional assay development using techniques such as multi-color flow cytometry, cytotoxicity assays, and cytokine quantitation
  • Viral vector transduction of primary human immune cells would be highly valued
  • Cellular immunotherapy experience preferred
  • Experience with tumor infiltrating lymphocytes (TILs) would be a plus
  • Authorized to work in the US at the time of application
Director/Senior Director of Process Development

Obsidian Therapeutics is pioneering controllable cell and gene therapies to deliver transformative outcomes for patients with intractable diseases. We are looking for a leader in CMC to build process development capabilities for Obsidian within the Technical Operations department driving the development of autologous and allogeneic cell therapy products in our pipeline targeting cancer. You will be a vital part of helping to achieve the Obsidian’s vision by collaboratively translating innovative science into medical breakthroughs for patients.

The Director/Senior Director of Process Development is a key leadership role within our Technical Operations function and will be responsible for establishing and leading the company’s process development capabilities. The individual in this role will work collaboratively with the Manufacturing, Analytical Development, Regulatory, and Quality functions to develop and tech transfer robust manufacturing processes and provide oversight of external manufacturing of viral vector and cell therapy drug substance/drug products to support Obsidian’s clinical pipeline. You will have significant opportunity to develop the strategy and execute on how Obsidian approaches the process development function and have an impact to Obsidian’s broader development strategies and efforts.

Responsibilities

The ideal candidate would have expertise in the development of clinical and commercial manufacturing processes for viral vector and cell therapy products, tech transfer to CDMOs, and oversight to external manufacturing. In, addition the candidate should have experience in managing teams and working with internal and external stakeholders to enable regulatory submissions.

  • Develop, tech transfer and implement cGMP manufacturing processes capable of supplying clinical trials with viral vector and cell therapy product (and related materials) meeting quality targets, and regulatory requirements
  • Identify and implement solutions to address current technical challenges in the manufacturing process and support immediate manufacturing needs and innovate to enable next generation process engineering
  • Develop and/or improve methods in collaboration with research and analytical development to characterize cell function relevant phenotype to support rigorous approach to evaluate impact of process changes
  • Supply development materials (viral vector, cell product) to support research/non-clinical studies, analytical development, and CDMO tech transfer activities
  • Author and review technical and scientific documents including but not limited to study protocols and reports, validation documents, SOPs, and test methods
  • Establish strong relationships and collaborate closely across multiple functions within Obsidian as well as externally with Obsidians strategic partners.
  • Collaborate effectively with research, CMC team, and CDMO to support IND enabling activities
  • Act as subject matter expert and represent the process development function as needed for regulatory interaction and author relevant CMC sections in regulatory documents
  • Recruit talent as needed and manage process development function with needed capabilities
  • Define agenda and manage experimental activities in the process development function and ensure proper documentation

Qualifications

  • D. in a relevant discipline (chemical engineering, biomolecular engineering, biotechnology or relevant field)
  • Minimum 10 years relevant industrial experience with prior direct experience in viral vectors and/or cell therapy required
  • Demonstrated track record with process development supporting early and late stage development of biologics (cell therapy, gene therapy, vaccines, antibodies…)
  • Experience with and technology transfer to CDMOs and external manufacturing oversight
  • Subject matter expertise in mammalian cell culture and related aspects such as cell metabolism/media optimization, engineering fundamentals on cell culture scale-up/down, cell biology/physiology
  • Prior experience with T-cells, NK cells, induced pluripotent stem cells (iPSC), or other primary cells is highly desirable
  • Familiar with product characterization methods relevant to immune cell-based product (e.g. flow cytometry, T-cell phenotyping, cell function characterization assays, PCR / qPCR, ELISA, and western blot) and/or viral vector manufacturing.
  • Familiar with FDA and EMA guidance documents relevant to gene and cell therapy
  • Experience authoring CMC sections of regulatory documents (IND/BLA) and engaging regulatory authorities.
  • Strong working knowledge of cGMP and quality systems requirements.
  • Ability to work in a fast-paced, collaborative environment, working with minimal direction and able to manage workload based on changing priorities.
  • Demonstrated leadership skills to build process development organization and the ability to collaborate with and effectively influence others.
  • Prior experience managing functional teams and representing function in CMC teams
Scientist/ Senior Scientist, Cell Line Development/Engineering

Obsidian Therapeutics is pioneering controllable cell and gene therapies to deliver transformative outcomes for patients with intractable diseases. We are looking for a scientist with strong background in cell line development to join Obsidian within the Technical Operations department and help engineer and develop stable clonal cell lines for viral vector production and/or novel feeders for cell therapy products.  You will be a vital part of helping to achieve the Obsidian’s vision by collaboratively translating innovative science into medical breakthroughs for patients with cancer.

Responsibilities

The ideal candidate will have expertise in all aspects of cell line engineering and development for use of such cell lines in GMP manufacturing processes for viral vector and/or cell therapy products with good understanding of regulatory requirements. This role will involve all molecular and cell biology aspects of cell line development from vector design/construction, cell culture maintenance, clone derivation, and analytical characterization.

  • Implement current approaches and associated technologies to establish clonal stable cell lines for production of viral vectors and/or use as feeders in cell therapy product
  • Drive creative solutions to ensure that high-throughput methods are applied to cell culture and analytical testing for selection of clones while ensuring that readouts from these methods predict performance at scale
  • Evaluate and confirm stability of generated cell lines and further characterize to meet all regulatory requirements and provide information for intended use in the manufacturing process
  • Plan and carry out experiments independently and effectively document all lab activities in notebooks in a timely manner
  • Support laboratory activities by monitoring lab equipment and maintaining a clean and safe working environment.
  • Communicate effectively through presentations and document work clearly through written reports
  • Serve as POC and oversee any outsourced cell line development projects including vendor identification, work plan development etc
  • Establish strong relationships and collaborate closely with Obsidian stakeholders (research, quality, regulatory, project management)
  • Represent cell line development in CMC sub-teams for assigned projects and support project activities including authoring of relevant CMC sections in regulatory documents

Required Qualifications

  • D., M.S., or B.S. in a relevant discipline (cell/molecular biology, biomolecular engineering, virology, biotechnology or equivalent) with 0-3 years (Ph.D.), 5-8 years (M.S./B.S.) relevant experience in cell line development required
  • Subject matter expertise in mammalian cell culture and related aspects such as cell metabolism/media optimization, engineering fundamentals on cell culture scale-up/down, cell biology/physiology, cell cloning as relevant to cell line development
  • Proficient in molecular cloning techniques, such as plasmid construction, RNA, cDNA and genomic DNA preparation, DNA electrophoresis, and Northern and Southern analyses.
  • Hands-on experience with analytical and cell characterization methods relevant to cell line development (e.g. flow cytometry, ELISA, Western, PCR).
  • Ability to work in a fast-paced, collaborative environment, working with minimal direction and able to manage workload based on changing priorities.

Preferred Qualifications

  • Experience working with production and characterization of viral vectors (lentiviral or retroviral)
  • Demonstrated track record with cell line development for use in GMP manufacturing of viral vector or other uses
  • Experience working with CDMOs for cell line development projects
  • Familiar with FDA and EMA guidance documents relevant to cell line development
  • Experience authoring CMC sections of regulatory documents (IND/BLA) and engaging regulatory authorities.
Scientist/ Senior Scientist, Cell Therapy Process Development

Obsidian Therapeutics is pioneering controllable cell and gene therapies to deliver transformative outcomes for patients with intractable diseases. We are looking for a scientist with strong background in cell therapy and/or process development to join Obsidian within the Technical Operations department and help drive the development of multiple cell therapy products in our pipeline targeting cancer. You will be a vital part of helping to achieve the Obsidian’s vision by collaboratively translating innovative science into medical breakthroughs for patients.

Responsibilities

The ideal candidate will have expertise to contribute to development of clinical/commercial manufacturing processes cell therapy products, tech transfer to CDMOs, oversight of external manufacturing, and regulatory filings.

  • Drive design and execution of experiments for development of the cGMP manufacturing process and associate process controls for clinical supply of cell therapy product (and related materials) meeting yield and quality targets
  • Author and review technical and scientific documents including but not limited to study protocols and reports, validation documents, SOPs, and test methods
  • Help build the team and mentor/train team members on experiment design, data analysis with adequate scientific rigor and proper documentation in lab notebooks
  • Innovate solutions to address current technical challenges and evaluate/ incorporate next generation process technologies
  • Support supply development materials (viral vector, cell product) to support research/non-clinical studies, analytical development, and CDMO tech transfer activities
  • Lead/support key technology transfer document preparation, technology transfer & implementation of the process, and serve as the technical point of contact for the process development function in assigned projects
  • Establish strong relationships and collaborate closely with Obsidian stakeholders (research, quality, regulatory, project management)
  • Represent process development in CMC sub-teams for assigned projects and support project activities including authoring of relevant CMC sections in regulatory documents

Required Qualifications

  • D. in a relevant discipline (chemical engineering, biomolecular engineering, biotechnology or relevant field)
  • Minimum 2-5 years relevant experience (industrial preferred) with prior direct experience in cell therapy required
  • Subject matter expertise in mammalian cell culture and related aspects such as cell metabolism/media optimization, engineering fundamentals on cell culture scale-up/down, cell biology/physiology
  • Prior experience with T-cells, NK cells, induced pluripotent stem cells (iPSC), or other primary cells is a plus.
  • Hands-on experience with product characterization methods relevant to cell therapy (e.g. flow cytometry).
  • Ability to work in a fast-paced, collaborative environment, working with minimal direction and able to manage workload based on changing priorities.

Preferred Qualifications

  • Demonstrated track record with process development supporting early and/or late stage development of biologics (cell therapy, gene therapy, vaccines, antibodies…)
  • Experience with and technology transfer to CDMOs and external manufacturing oversight
  • Familiar with FDA and EMA guidance documents relevant to gene and cell therapy
  • Experience authoring CMC sections of regulatory documents (IND/BLA) and engaging regulatory authorities.
  • Strong working knowledge of cGMP and quality systems requirements.
  • Prior experience in managing teams
Associate Scientist/ Senior Associate Scientist, Cell Therapy Process Development

About the Company:

Obsidian Therapeutics is a biotechnology company that is pioneering the development of controllable cell and gene therapies to deliver transformative outcomes for patients with intractable diseases.  Obsidian’s programs apply our CytoDriveTM technology in Cell and Gene therapy products to control expression of proteins for enhanced therapeutic efficacy and safety. 

Scope:

We are looking for a highly motivated Associate Scientist or Senior Associate Scientist with expertise in cell culture and upstream process development to join the technical operations department at Obsidian and develop and characterize manufacturing processes for cell therapy pipeline products.  The successful candidate will be part of a CMC team will work collaboratively with the research groups to transfer foundational knowhow and build on it to deliver phase-appropriate manufacturing process that meets the quality target profile, to bring life-saving products to the clinic. This role is a unique opportunity to be a scientific contributor in a well-funded startup with blue chip investors, field-leading founders and advisors, and a highly experienced startup team. You will help us build a dynamic, passionate, collaborative, transparent and successful organization focused on delivering transformative therapies in areas of greatest clinical need. Located in an energetic environment in the heart of Cambridge, Obsidian is an equal opportunity employer offering a competitive salary and benefits package. Resumes can be sent to jobs@obsidiantx.com.

Key Responsibilities:

  • Design, plan, and execute process development experiments with engineered T-cell and other immune cell product candidates to define process, optimize and scale-up for clinical manufacturing encompassing all steps of the process including starting material isolation, expansion, viral transduction, and cryopreservation.
  • Perform in in process assays to measure cell number and characterize product using flow cytometry and other techniques
  • Interpret results, troubleshoot technical hurdles, and propose solutions to the team
  • Maintain excellent records of experiments in lab notebooks in a timely manner
  • Collaborate effectively across Obsidian organization, present internally to project teams, management, and scientific teams
  • Author and review technical reports detailing experimental work and summarizing results
  • Maintain a safe work environment in accordance with policies/procedures/regulations
  • Maintain cell cultures and cell banks as needed to support experimental work including occasional production of viral vectors
  • Support lab functions-including, but not limited to restocking consumables, maintaining equipment and cleaning

Minimum Qualifications:

  • S. or M.S. degree in chemical engineering, biochemical engineering, biology or relevant field with 5+ years of experience (M.S.) or 8+ years of experience (B.S.) in biotech/pharma setting
  • Strong experience with cell culture techniques with both adherent and suspension cell lines
  • Hands-on experience with plasmid transfection and viral transduction using lentiviral/retroviral vectors
  • Analytical methods for cell characterization such as flow cytometry, quantitative PCR, western blot, and ELISA
  • Strong troubleshooting and communication skills, highly organized with strong record keeping and an ability to multi-task
  • Ability to work independently and as part of teams in a fast-paced team environment, meet deadlines, prioritize work across multiple projects

Preferred Qualifications:

  • Experience with T-cell (or other immune cell) selection, viral transduction, gene editing technologies, cell expansion and cryopreservation
  • Basic knowledge of immunology and experience working with immune system cells
  • Experience with design of experiment (DOE) approaches and statistical analysis of data
  • Experience with culture of mammalian cells in small -scale and large scale bioreactor systems and associated control strategies and automation
  • Prior experience working with CDMOs, technology transfer in context of process development to support GMP manufacturing
Scientist/ Senior Scientist, High Throughput Biology (Discovery)

About the Role:

At Obsidian Therapeutics, we are pioneering the development of controllable cell and gene therapies to deliver transformative outcomes for patients with intractable diseases.  Obsidian’s programs apply our cytoDRiVETM technology in Cell and Gene therapy products to control expression of proteins for enhanced therapeutic efficacy and safety.

As a Scientist in High Throughput Biology, you will work as part of the Discovery group to accelerate Obsidian’s therapeutic pipeline using state-of-the-art high throughput screening approaches to identify new target opportunities and to optimize cytoDRiVETM control of therapeutic target.  In this role, you will be responsible for executing all aspects of screening campaigns including construction of combinatorial cDNA libraries, assay development, functional screening, and data analysis.  You will work independently and as part of cross functional teams comprised of immunologists, cell biologists, and molecular biologists dedicated to designing and delivering controllable cellular therapies to patients.

This role is a unique opportunity to be a scientific contributor in a well-funded startup with blue chip investors, field-leading founders and advisors, and a highly experienced startup team. You will help us build a dynamic, passionate, collaborative, transparent and successful organization focused on delivering transformative therapies in areas of greatest clinical need. Located in an energetic environment in the heart of Cambridge, Obsidian is an equal opportunity employer offering a competitive salary and benefits package. Resumes can be sent to jobs@obsidiantx.com.

Key Responsibilities:

  • Design and build libraries for functional screening, with a focus on combinatorial cDNA libraries working with our in-house molecular biology teams and CROs
  • Develop and trouble-shoot high throughput functional assays in disease relevant cell models.
  • Execute high throughput screens, nominate hits, and design strategies for hit validation and follow-up in collaboration with cross functional project teams.
  • Work with the computational biology team to analyze and interpret data.
  • Maintain accurate lab notebooks, present data to project teams, management and as part of external collaborations.

Required Qualifications & Skills

  • D. or equivalent in cell biology, genomics, biological engineering, or a related field with 2+ years of experience in a biotech/pharma setting.
  • Demonstrated expertise in high throughput screening using functional genomics approaches, including cDNA, CRISPR, or RNAi based methods in pooled or arrayed formats.
  • Technical expertise in all aspects of functional genomics screening including delivery methods, analytical tools, automation, and data analysis
  • Experience with lenti-viral packaging, transduction, and associated analytical methods is essential
  • Strong troubleshooting and communication skills, highly organized with strong record keeping and an ability multi-task
  • Highly collaborative working style and an ability to adapt in a fast paced, rapidly developing environment

Preferred Qualifications & Skills:

  • Experience with next generation sequencing for pooled barcode screening, including library generation and data analysis.
  • Knowledge of the cell and gene therapy field is highly desirable.
  • Experience working with primary immune cells is a plus
  • Previous experience in synthetic biology, engineering gene circuits, or regulated gene expression
Associate Director, High Throughput Biology (Discovery)

About the Company:

At Obsidian Therapeutics, we are pioneering the development of controllable cell and gene therapies to deliver transformative outcomes for patients with intractable diseases.  Obsidian’s programs apply our cytoDRiVETM technology in Cell and Gene therapy products to control expression of proteins for enhanced therapeutic efficacy and safety.

We seek an accomplished screening scientist and enthusiastic leader to join the Discovery group as Associate Director of the newly formed High Throughput Biology team.  In this role, you will build and lead a team applying state of the art functional screening technologies to identify new target opportunities and optimize the design of lead candidates to accelerate Obsidian’s therapeutic pipeline.  You will be responsible for leading a team responsible for all aspects of our screening platform, including constructing combinatorial cDNA libraries, developing high throughput screen assays in T-cells and other primary cell types, and the execution of screening campaigns including data analysis and interpretation.  You will work as part of cross functional teams comprised of immunologists, cell biologists, and molecular biologists dedicated to designing and delivering controllable cellular therapies to patients.

This role is a unique opportunity to be a scientific contributor in a well-funded startup with blue chip investors, field-leading founders and advisors, and a highly experienced startup team. You will help us build a dynamic, passionate, collaborative, transparent and successful organization focused on delivering transformative therapies in areas of greatest clinical need. Located in an energetic environment in the heart of Cambridge, Obsidian is an equal opportunity employer offering a competitive salary and benefits package. Resumes can be sent to jobs@obsidiantx.com.

Key Responsibilities:

  • Manage all aspects of screening campaigns from inception, through assay development, screening, data analysis, and follow-up
  • Provide scientific oversight and strategic leadership to the High Throughput Biology team
  • Represent the High Throughput Screening group as part of internal project teams, management teams, and in external collaborations.
  • Work with other members of the discovery steam to manage the department, provide mentorship and growth of staff, and maximize our impact on the Obsidian’s pipeline
  • Collaborate effectively across Obsidian organization, present internally to project teams, management and scientific teams.

Required Qualifications & Skills

  • PhD in cell biology, genomics, biological engineering, or a related field with 5+ years of experience in a biotech/pharma setting.
  • Demonstrated expertise in high throughput screening using functional genomics approaches, including cDNA, CRISPR, and/or siRNA-based methods in both pooled and arrayed formats.
  • Technical expertise in all aspects of functional genomics screening including delivery methods, analytical tools, instrumentation, and data analysis
  • Experience with next generation sequencing for pooled barcode screening, including library generation and data analysis.
  • Demonstrated management expertise, track record of leading high performing teams, and a commitment to mentorship and development of team members
  • Experience working with external vendors for molecular biology & genomics
  • Strong troubleshooting and communication skills, highly organized with strong record keeping and an ability multi-task
  • Highly collaborative working style and ability to adapt in a fast paced, rapidly developing environment

Preferred Qualifications & Skills:

  • Experience with genome editing and cell engineering using homology directed repair
  • Familiarity with current automation and screening instrumentation
  • Knowledge of the cell and gene therapy field is highly desirable.
  • Experience with functional screening in immune cell types
  • Previous experience in synthetic biology, engineering gene circuits, or regulated gene expression
Senior Research Associate, Molecular Assays

About the Role:

At Obsidian Therapeutics, we are pioneering the development of controllable cell and gene therapies to deliver transformative outcomes for patients with intractable diseases.  Obsidian’s programs apply our cytoDRiVETM technology in Cell and Gene therapy products to control expression of proteins for enhanced therapeutic efficacy and safety.

As a Senior Research Associate in Molecular assays, you will work as part of the Discovery group to accelerate Obsidian’s therapeutic pipeline using state-of-the-art high throughput screening approaches to identify new target opportunities and the design of therapeutic lead candidates to optimize cytoDRiVETM control.  In this role, you will be responsible for the design, planning, and execution of next generation sequencing experiments and analytical methods aimed at characterizing cytoDRiVETM circuits.  You will work independently and as part of a cross functional discovery teams comprised of immunologists, cell biologists, and molecular biologists dedicated to designing and delivering controllable cellular therapies to patients.

This role is a unique opportunity to be a scientific contributor in a well-funded startup with blue chip investors, field-leading founders and advisors, and a highly experienced startup team. You will help us build a dynamic, passionate, collaborative, transparent and successful organization focused on delivering transformative therapies in areas of greatest clinical need. Located in an energetic environment in the heart of Cambridge, Obsidian is an equal opportunity employer offering a competitive salary and benefits package. Resumes can be sent to jobs@obsidiantx.com.

Key Responsibilities:

  • Plan, design, execute and improve key assays in our cytoDRiVETM screening platform with a focus on the broad use of NGS and other analytical methods
  • Collaborate with the computational biology team for analysis and interpretation of NGS data that directly support research activities, project goals, and platform development
  • Develop, implement, and trouble-shoot automation protocols, including training staff in the use of automation protocols
  • Manage relationships with CROs and external vendors
  • Maintain accurate lab notebooks, present data to project teams, management and scientific teams

Required Qualifications & Skills

  • Bachelor’s degree or equivalent in molecular biology, biochemistry, genomics, biological engineering, or a related field with 5+ years of experience in a biotech/pharma setting.
  • Demonstrated expertise in all aspects of NGS workflows including library preparation, sequencing, and post-processing using the Illumina platform is required
  • Prior experience developing automated workflows for molecular biology and sequencing
  • Strong troubleshooting and communication skills, highly organized and detail oriented with strong record keeping and an ability multi-task
  • Highly collaborative working style and an ability to adapt in a fast paced, rapidly developing environment

Preferred Qualifications & Skills:

  • Experience pooled barcode screening, including library generation and data analysis
  • Knowledge of the cell and gene therapy field is highly desirable
  • Experience working with cell culture techniques is a plus
  • Understanding of LIMs and experience working with data integration into electronic notebooks such as Benchling
Senior Scientist, Computational Biology (Discovery)

About the Role:

At Obsidian Therapeutics, we are pioneering the development of controllable cell and gene therapies to deliver transformative outcomes for patients with intractable diseases.  Obsidian’s programs apply our cytoDRiVETM technology in Cell and Gene therapy products to control expression of proteins for enhanced therapeutic efficacy and safety.

As a Senior Scientist in Computational Biology, you will work to accelerate Obsidian’s therapeutic pipeline using computational approaches to identify new target opportunities and optimize design of therapeutic lead candidates for cytoDRiVETM control of cell and gene therapies.  In this role, you will build data analysis pipelines, analyze screening data sets to formulate therapeutic hypothesis, and integrate our internal discovery data with information from public databases to formulate therapeutic hypotheses and guide patient selection strategies for our therapeutic programs.  You will work as part of cross functional teams comprised of immunologists, cell biologists, and molecular biologists dedicated to designing and delivering controllable cellular therapies to patients.

This role is a unique opportunity to be a scientific contributor in a well-funded startup with blue chip investors, field-leading founders and advisors, and a highly experienced startup team. You will help us build a dynamic, passionate, collaborative, transparent and successful organization focused on delivering transformative therapies in areas of greatest clinical need. Located in an energetic environment in the heart of Cambridge, Obsidian is an equal opportunity employer offering a competitive salary and benefits package. Resumes can be sent to jobs@obsidiantx.com.

 

Key Responsibilities:

  • Collaborate with the High Throughput Biology team on the design and analysis of functional screens to support target identification, lead optimization, and platform development
  • Identify, analyze, and integrate public datasets with internally generated screening data to contextualize internal discovery data and formulate therapeutic hypotheses.
  • Modelling to inform the design of cytoDRiVETM control systems and to guide data analysis on system performance
  • Work cross functionally with project teams to design studies that are appropriately powered with the proper controls to meet experimental and business objectives
  • Partner with the information technology team to build Obsidian’s core informatics infrastructure and establish automated analysis pipelines to integrate with laboratory equipment, LIMS and ELN.

 

Required Qualifications & Skills

  • D. in computational biology, bioinformatics, engineering, physics, or a related discipline with 2+ years of experience in a biotech/pharma setting or a bachelor’s degree with equivalent experience
  • Proficiency in R or Python, and experience using software libraries for data processing, statistical analysis, and data visualization is essential
  • Demonstrated expertise in implementing workflows to process high-throughput sequencing data, and performing downstream analysis to generate knowledge and create biological insight
  • Strong troubleshooting and communication skills, highly organized and detail oriented with strong record keeping and an ability multi-task
  • Highly collaborative working style and an ability to adapt in a fast paced, rapidly developing environment

 

Preferred Qualifications & Skills:

  • Previous experience analyzing pooled barcode screening data, including library generation and data analysis.
  • Knowledge in cell and gene therapy or immunology is highly desirable
  • Experience developing data infrastructure and integration with ELN platforms is desirable
  • Familiarity with protein structure analysis using Rosetta or similar software packages is a plus
  • Familiarly with software development best practices